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. 1972 Feb;126(4):965–973. doi: 10.1042/bj1260965

Metabolism of [14C]adenine and derivatives by cerebral tissues, superfused and electrically stimulated

I Pull 1, H McIlwain 1
PMCID: PMC1178504  PMID: 5073246

Abstract

1. Uptake of [14C]adenine and [14C]adenosine from surrounding fluids to guinea-pig cerebral tissues was measured during incubation in vitro. Output of 14C-labelled compounds from the loaded tissues to superfusion fluids occurred on continued incubation, at about 0.2% of the tissue's content/min, and this rate was increased about fourfold by electrical excitation of the tissue. 2. The compounds released from the tissue to superfusion fluids included adenine, adenosine, inosine and hypoxanthine with small amounts of nucleotides. Output of all these compounds, except adenine, increased on excitation. Media depleted of oxygen or glucose also increased the output of 14C-labelled derivatives from [14C]adenine-loaded tissues, and this augmented output was further increased by electrical stimulation. 3. [14C]Adenosine was found as the main product from [14C]ATP when this was added at low concentrations to fluids superfusing cerebral tissue. Metabolic and neurohumoural explanations of the liberation and action of adenosine derivatives in the tissue are discussed.

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Selected References

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  1. Antonis A., Clark M., Pilkington T. R. A semiautomated fluorimetric method for the enzymatic determination of pyruvate, lactate, acetoacetate, and beta-hydroxybutyrate levels in plasma. J Lab Clin Med. 1966 Aug;68(2):340–356. [PubMed] [Google Scholar]
  2. Burnstock G., Campbell G., Satchell D., Smythe A. Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic inhibitory nerves in the gut. Br J Pharmacol. 1970 Dec;40(4):668–688. doi: 10.1111/j.1476-5381.1970.tb10646.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Douglas W. W., Poisner A. M. On the relation between ATP splitting and secretion in the adrenal chromaffin cell: extrusion of ATP (unhydrolysed) during release of catecholamines. J Physiol. 1966 Mar;183(1):249–256. doi: 10.1113/jphysiol.1966.sp007864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ESSIG C. F., GROCE M. E., WILLIAMSON E. L. Reversible elevation of electroconvulsive threshold and occurrence of spontaneous convulsions upon repeated electrical stimulation of the cat brain. Exp Neurol. 1961 Jul;4:37–47. doi: 10.1016/0014-4886(61)90076-0. [DOI] [PubMed] [Google Scholar]
  5. Geffen L. B., Livett B. G. Synaptic vesicles in sympathetic neurons. Physiol Rev. 1971 Jan;51(1):98–157. doi: 10.1152/physrev.1971.51.1.98. [DOI] [PubMed] [Google Scholar]
  6. HOLTON F. A., HOLTON P. The capillary dilator substances in dry powders of spinal roots; a possible role of adenosine triphosphate in chemical transmission from nerve endings. J Physiol. 1954 Oct 28;126(1):124–140. doi: 10.1113/jphysiol.1954.sp005198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOLTON P. The liberation of adenosine triphosphate on antidromic stimulation of sensory nerves. J Physiol. 1959 Mar 12;145(3):494–504. doi: 10.1113/jphysiol.1959.sp006157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ipata P. L. Sheep brain 5'-nucleotidase. Some enzymic properties and allosteric inhibition by nucleoside triphosphates. Biochemistry. 1968 Feb;7(2):507–515. doi: 10.1021/bi00842a004. [DOI] [PubMed] [Google Scholar]
  9. JORDAN W. K., MARCH R., HOUCHIN O. B., POPP E. Intracellular partition of purine deaminases in rodent brain. J Neurochem. 1959 Jun;4(2):170–174. doi: 10.1111/j.1471-4159.1959.tb13187.x. [DOI] [PubMed] [Google Scholar]
  10. Kakiuchi S., Rall T. W., McIlwain H. The effect of electrical stimulation upon the accumulation of adenosine 3',5'-phosphate in isolated cerebral tissue. J Neurochem. 1969 Apr;16(4):485–491. doi: 10.1111/j.1471-4159.1969.tb06847.x. [DOI] [PubMed] [Google Scholar]
  11. Katz R. I., Chase T. N. Neurohumoral mechanisms in the brain slice. Adv Pharmacol Chemother. 1970;8:1–30. doi: 10.1016/s1054-3589(08)60592-x. [DOI] [PubMed] [Google Scholar]
  12. McIlwain H., Snyder S. H. Stimulation of piriform- and neo-cortical tissues in an in vitro flow-system: metabolic properties and release of putative neurotransmitters. J Neurochem. 1970 Apr;17(4):521–530. doi: 10.1111/j.1471-4159.1970.tb00530.x. [DOI] [PubMed] [Google Scholar]
  13. Santos J. N., Hempstead K. W., Kopp L. E., Miech R. P. Nucleotide metabolism in rat brain. J Neurochem. 1968 May;15(5):367–376. doi: 10.1111/j.1471-4159.1968.tb11623.x. [DOI] [PubMed] [Google Scholar]
  14. Sattin A., Rall T. W. The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices. Mol Pharmacol. 1970 Jan;6(1):13–23. [PubMed] [Google Scholar]
  15. Setlow B., Lowenstein J. M. Adenylate deaminase. II. Purification and some regulatory properties of the enzyme from calf brain. J Biol Chem. 1967 Feb 25;242(4):607–615. [PubMed] [Google Scholar]
  16. Shimizu H., Creveling C. R., Daly J. Stimulated formation of adenosine 3',5'-cyclic phosphate in cerebral cortex: synergism between electrical activity and biogenic amines. Proc Natl Acad Sci U S A. 1970 Apr;65(4):1033–1040. doi: 10.1073/pnas.65.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shimizu H., Daly J. Formation of cyclic adenosine 3',5'-monophosphate from adenosine in brain slices. Biochim Biophys Acta. 1970 Nov 24;222(2):465–473. doi: 10.1016/0304-4165(70)90137-6. [DOI] [PubMed] [Google Scholar]
  18. Siggins G. R., Hoffer B. J., Bloom F. E. Cyclic adenosine monophosphate: possible mediator for norepinephrine effects on cerebellar Purkinje cells. Science. 1969 Sep 5;165(3897):1018–1020. doi: 10.1126/science.165.3897.1018. [DOI] [PubMed] [Google Scholar]

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