Figure 2. scRNA-seq of intratumoral immune cells from Y1.7LI melanoma-bearing mice treated with neoAg SLP vaccines or ICT.

(A) Y1.7LI melanoma-bearing mice were treated as indicated beginning on day 7 post-tumor transplant. Tumors from individual mice were harvested on day 15, pooled, and processed, and live CD45⁺ cells were sorted and analyzed by scRNA-seq.

(B) Uniform manifold approximation and projection (UMAP) plot from scRNA-seq of intratumoral CD45⁺ cells with annotated cell types.

(C) Feature plot showing lineage-specific transcripts.

(D) Feature plots displaying subclustering of activated T cell-containing clusters.

(E) Heatmap displaying average expression of select transcripts by cluster.

(F) Frequency of subclustered T cell-containing clusters by treatment. See also Figure S4 and Table S1.