Table II.

Cellular Attributes Measured to Assess Cytotoxicity and Definition of Abnormality Threshold Used in the Data Analysis

#	Attributes	Definition of the abnormality threshold
1	% non-adherent cells	(Mean cell number in well of untreated cells – mean cell number of wells with treated cells) / mean cell number in well of untreated cells) * 100
2	Elevated mitochondrial activity	Single-cell fluorescence intensity (FI) of the mitochondrial activity stain in the treatment group is > 2 SD of the mean FI of the untreated population
3	Reduced mitochondrial activity	Single-cell FI of the mitochondrial activity stain in the treatment group is < 2 SD of the mean FI of the untreated population
4	Elevated membrane permeability	Single-cell FI of the membrane integrity stain in the treatment group is > 2 SD of the mean FI of the untreated population
5	Abnormal nuclear area	Single-cell area of the nuclear stain in the treatment group is either > or < 2 SD of the mean nuclear area of the untreated population
6	Elevated cellular area	Single-cell area of the cytoplasm stain in the treatment group is either > 2 SD of the mean cellular area of the untreated population
7	Polynucleated cells	(Mean number of polynucleated cells in untreated cells –number of polynucleated cells in well of treated cells) / mean number of polynucleated cells in untreated cells) * 100
8	Elevated vacuole area	Single-cell area of the non-stained cytoplasm (vacuole space) in the treatment group > 2 SD of the area of the non-stained cytoplasm (vacuole space) of the untreated population
9	Elevated neutral lipids	Single-cell FI of the neutral lipid stain in the treatment group is > 2 SD of the mean FI of the untreated population
10	Elevated phospholipids	Single-cell FI of the phospholipid stain in the treatment group is > 2 SD of the mean FI of the untreated population