Table III.

Attributes of Untreated J774A.1 Cells and hAMs Derived from Image Analysis. The Data was Calculated from n = 20 Experiments (with Six Technical Replicates Each) for J774A.1 Cells (Passage Numbers #8–30) and 5 Different hAM Donors (D1-5; with Six Technical Replicates Each)

Attribute	J774.A1					hAM
	Mean	2SD	RSD%	Min	Max	Mean	2SD	RSD%	Min	Max
Cell number per well	1002	752	38	103	2138	260	224	43	109	472
FI (a.u.) MitoTracker®: mitochondrial activity	7126	4872	34	52	12876	14436	13920	48	7496	25765
FI (a.u.) Image-IT™ DEAD GreenTM: membrane integrity	5760	4252	37	3181	10637	6599	6424	49	2475	10104
Nuclear area (µm2)	101	38	19	75	135	94	16	9	84	105
Cell area (µm2)	249	116	23	137	408	338	106	16	252	393
Number of polynucleated cells / well	25	42	84	5	105	9	10	56	3	15
Vacuole area (µm2)	4	18	225	0	35	16	68	213	0	77
FI (a.u.) LipidTOX™ Green: neutral lipids	6453	6886	53	3404	16738	11492	5500	24	8645	15757
FI (a.u.) LipidTOX™ Red: phospholipids	2789	5148	92	0	9972	4343	6938	80	1448	10311