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. 1972 Apr;127(2):375–385. doi: 10.1042/bj1270375

Metabolism of fatty acids by bovine spermatozoa

A R Neill 1,2, C J Masters 1,2
PMCID: PMC1178598  PMID: 4342556

Abstract

The incorporation of 14C-labelled myristic, palmitic, stearic, oleic and linoleic acids in vitro into the lipids of bovine spermatozoa was measured at intervals from 2min to 2h. All acids were rapidly incorporated into diglycerides, myristic acid being metabolized to the greatest extent. Whereas the low incorporation of acids into total phospholipids reflected the relative stability of the major phospholipid fractions in sperm, the minor phospholipids, particularly phosphatidylinositol, showed comparatively high metabolic activity. Although, in general, saturated acids were incorporated more actively than unsaturated substrates, stearic acid was poorly incorporated into all lipids except phosphatidylinositol. In regard to fatty acid composition of sperm lipids it was notable that diglycerides contained myristic acid as the major component, and this acid was also a prominent moiety of phosphatidylinositol. Docosahexaenoic acid was the principal fatty acid of the major phospholipid classes. These findings have been discussed in relation to the role of lipids in the metabolism of spermatozoa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AAES-JORGENSEN E. Essential fatty acids. Physiol Rev. 1961 Jan;41:1–51. doi: 10.1152/physrev.1961.41.1.1. [DOI] [PubMed] [Google Scholar]
  2. DAWSON R. M. Studies on the enzymic hydrolysis of monophosphoinositide by phospholipase preparations from P. notatum and ox pancreas. Biochim Biophys Acta. 1959 May;33(1):68–77. doi: 10.1016/0006-3002(59)90499-8. [DOI] [PubMed] [Google Scholar]
  3. DAWSON R. M. The labelling of ram semen in vivo with radioactive phosphate and [carboxy-14C] stearic acid. Biochem J. 1958 Mar;68(3):512–519. doi: 10.1042/bj0680512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis J. T., Bridges R. B., Coniglio J. G. Changes in lipid composition of the maturing rat testis. Biochem J. 1966 Jan;98(1):342–346. doi: 10.1042/bj0980342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  6. GRAY G. M. The phospholipids of ox spleen with special reference to the fatty acid and fatty aldehyde compositions of the lecithin and kephalin fractions. Biochem J. 1960 Oct;77:82–91. doi: 10.1042/bj0770082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HARTREE E. F., MANN T. Phospholipids in ram semen: metabolism of plasmalogen and fatty acids. Biochem J. 1961 Sep;80:464–476. doi: 10.1042/bj0800464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOLMAN R. T., HOFSTETTER H. H. THE FATTY ACID COMPOSITION OF THE LIPIDS FROM BOVINE AND PORCINE REPRODUCTIVE TISSUES. J Am Oil Chem Soc. 1965 Jun;42:540–544. doi: 10.1007/BF02540098. [DOI] [PubMed] [Google Scholar]
  9. JAMES A. T. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. Methods Biochem Anal. 1960;8:1–59. doi: 10.1002/9780470110249.ch1. [DOI] [PubMed] [Google Scholar]
  10. KARNOVSKY M. L., WALLACH D. F. The metabolic basis of phagocytosis. III. Incorporation of inorganic phosphate into various classes of phosphatides during phagocytosis. J Biol Chem. 1961 Jul;236:1895–1901. [PubMed] [Google Scholar]
  11. KEMP P., HUBSCHER G., HAWTHORNE J. N. Phosphoinositides. 3. Enzymic hydrolysis of inositol-containing phospholipids. Biochem J. 1961 Apr;79:193–200. doi: 10.1042/bj0790193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KENNEDY E. P. Metabolism of lipides. Annu Rev Biochem. 1957;26:119–148. doi: 10.1146/annurev.bi.26.070157.001003. [DOI] [PubMed] [Google Scholar]
  13. Mills S. C., Scott T. W. Metabolism of fatty acids by testicular and ejaculated ram spermatozoa. J Reprod Fertil. 1969 Mar;18(2):367–369. doi: 10.1530/jrf.0.0180367. [DOI] [PubMed] [Google Scholar]
  14. Neill A. R., Masters C. J. Incorporation of (U-14C)palmitic acid into the phospholipids of bovine semen. J Reprod Fertil. 1971 Feb;24(2):295–297. doi: 10.1530/jrf.0.0240295. [DOI] [PubMed] [Google Scholar]
  15. Owens K. A two-dimensional thin-layer chromatographic procedure for the estimation of plasmalogens. Biochem J. 1966 Aug;100(2):354–361. doi: 10.1042/bj1000354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Payne E., Masters C. J. The incorporation of fatty acids into ruminant erythrocytes in vitro. Comp Biochem Physiol. 1969 May;29(2):717–729. doi: 10.1016/0010-406x(69)91623-5. [DOI] [PubMed] [Google Scholar]
  17. Pursel V. G., Graham E. F. Phospholipids of bovine spermatozoa and seminal plasma. J Reprod Fertil. 1967 Oct;14(2):203–211. doi: 10.1530/jrf.0.0140203. [DOI] [PubMed] [Google Scholar]
  18. ROOSEN-RUNGE E. C. Quantitative investigations on human testicular biopsies. I. Normal testis. Fertil Steril. 1956 May-Jun;7(3):251–261. doi: 10.1016/s0015-0282(16)32344-5. [DOI] [PubMed] [Google Scholar]
  19. Scott T. W., Dawson R. M. Metabolism of phospholipids by spermatozoa and seminal plasma. Biochem J. 1968 Jul;108(3):457–463. doi: 10.1042/bj1080457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scott T. W., Hansel W., Donaldson L. E. Metabolism of phospholipids and the characterization of fatty acids in bovine corpus luteum. Biochem J. 1968 Jun;108(2):317–323. doi: 10.1042/bj1080317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scott T. W., Mills S. C., Freinkel N. The mechanism of thyrotrophin action in relation to lipid metabolism in thyroid tissue. Biochem J. 1968 Sep;109(3):325–332. doi: 10.1042/bj1090325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scott T. W., Voglmayr J. K., Setchell B. P. Lipid composition and metabolism in testicular and ejaculated ram spermatozoa. Biochem J. 1967 Feb;102(2):456–461. doi: 10.1042/bj1020456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TERNER C., KORSH G. The biosynthesis of C-14-labeled lipids by isolated bull spermatozoa. Biochemistry. 1962 Mar;1:367–372. doi: 10.1021/bi00908a027. [DOI] [PubMed] [Google Scholar]
  24. VIGNAIS P. M., VIGNAIS P. V., LEHNINGER A. L. IDENTIFICATION OF PHOSPHATIDYLINOSITOL AS A FACTOR REQUIRED IN MITOCHONDRIAL CONTRACTION. J Biol Chem. 1964 Jun;239:2011–2021. [PubMed] [Google Scholar]
  25. Viswanathan C. V., Phillips F., Lundberg W. O. Two-dimensional reaction thin-layer chromatography in the analysis of phosphatide plasmalogens. J Chromatogr. 1968 May 21;35(1):66–71. doi: 10.1016/s0021-9673(01)82350-5. [DOI] [PubMed] [Google Scholar]

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