Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1972 Apr;127(2):10P–11P. doi: 10.1042/bj1270010p

The separation of functional and non-functional xanthine oxidase by affinity chromatography.

V Massey, D Edmondson, G Palmer, L M Beacham 3rd, G B Elion
PMCID: PMC1178622  PMID: 4672853

Full text

PDF
10P

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAY R. C., PETTERSSON R., EHRENBERG A. The chemistry of xanthine oxidase. 7. The anaerobic reduction of xanthine oxidase studied by electron-spin resonance and magnetic susceptibility. Biochem J. 1961 Oct;81:178–189. doi: 10.1042/bj0810178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coughlan M. P., Rajagopalan K. V., Handler P. The role of molybdenum in xanthine oxidase and related enzymes. Reactivity with cyanide, arsenite, and methanol. J Biol Chem. 1969 May 25;244(10):2658–2663. [PubMed] [Google Scholar]
  3. Cuatrecasas P., Wilchek M., Anfinsen C. B. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636–643. doi: 10.1073/pnas.61.2.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MORELL D. B. The nature and catalytic activities of milk xanthine oxidase. Biochem J. 1952 Aug;51(5):657–666. doi: 10.1042/bj0510657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Massey V., Brumby P. E., Komai H. Studies on milk xanthine oxidase. Some spectral and kinetic properties. J Biol Chem. 1969 Apr 10;244(7):1682–1691. [PubMed] [Google Scholar]
  6. Massey V., Edmondson D. On the mechanism of inactivation of xanthine oxidase by cyanide. J Biol Chem. 1970 Dec 25;245(24):6595–6598. [PubMed] [Google Scholar]
  7. Massey V., Komai H., Palmer G., Elion G. B. On the mechanism of inactivation of xanthine oxidase by allopurinol and other pyrazolo[3,4-d]pyrimidines. J Biol Chem. 1970 Jun 10;245(11):2837–2844. [PubMed] [Google Scholar]
  8. Massey V., Komai H., Palmer G., Elion G. B. The existence of nonfunctional active sites in milk xanthine oxidase: reaction with functional active site inhibitors. Vitam Horm. 1970;28:505–531. doi: 10.1016/s0083-6729(08)60909-7. [DOI] [PubMed] [Google Scholar]
  9. McGartoll M. A., Pick F. M., Swann J. C., Bray R. C. Properties of xanthine oxidase preparations dependent on the proportions of active and inactivated enzyme. Biochim Biophys Acta. 1970 Sep 16;212(3):523–526. doi: 10.1016/0005-2744(70)90264-0. [DOI] [PubMed] [Google Scholar]
  10. Spector T., Johns D. G. Stoichiometric inhibition of reduced xanthine oxidase by hydroxypyrazolo [3,4-d]pyrimidines. J Biol Chem. 1970 Oct 10;245(19):5079–5085. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES