Table 3. Transfection efficiency of CRISPR/Cas9 system in NK cells.

Cell source	Genetic modification system	Genetic material	Transfection efficiency	Reference
Human primary NK cells	CRISPR/Cas9 (electroporation)	RNP for NKG2A KO	13.8%	[39]
Human primary NK cells	CRISPR/Cas9 (electroporation)	RNP for CALHM2 KO	~40%	[41]
NK92	CRISPR/Cas9 (electroporation)	RNP for CALHM2 KO	~60%	[41]
Mouse primary NK cells	CRISPR/Cas9 (electroporation)	RNP for CALHM2 KO	~70%	[41]
Human primary NK cells	CRISPR/Cas9 and TcB transposon system (electroporation)	RNP for CISH KO	~50%	[40]

Abbreviations: clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9); NK, natural killer; RNP, ribonucleoprotein; KO, knockout; TcB; TcBuster.