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. 2024 Dec 5;10(2):e184711. doi: 10.1172/jci.insight.184711

Figure 2. 89.6 VT1 identifies reversible latency in primary human HSPCs.

Figure 2

(A) Schematic of the experimental process for B–E. (B) Flow cytometric analysis of HSPCs expanded, transduced, and sorted according to the timeline shown in A. As indicated, actively infected cells were removed via FACS by sorting latently infected (GFP+mCherry–) cells. The isolated cells were mixed with uninfected cells so that changes in the proportions of active and latent infection following LRA treatment could be more accurately quantified. (C and D) Flow cytometric analysis of HSPCs from B divided into 37°C or 30°C incubation conditions with LRAs as indicated for 24 hours. (E) Summary graph of flow cytometric analysis (C and D). Result is shown for 1 experiment. (F) Schematic of the experimental process for G and H. (G) Flow cytometric analysis of HSPCs expanded and transduced according to the timeline shown in F. (H) Summary graph of flow cytometric analysis performed as in G. Statistical significance was determined by 2-way ANOVA with Holm-Šídák multiple comparisons test. The mean ± standard deviation is shown for 3 independent experiments. ****P ≤ 0.0001. SSC, side scatter.