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. 1970 May;117(5):939–946. doi: 10.1042/bj1170939

Studies on mammalian glucoamylases with special reference to monkey intestinal glucoamylase

B Seetharam 1, N Swaminathan 1,*, A N Radhakrishnan 1
PMCID: PMC1179053  PMID: 4989041

Abstract

1. Highly purified preparations of glucoamylase were obtained from liver, spleen and intestine of the monkey. The enrichment factor was lower for intestine (60-fold) compared with that of liver (1200-fold) and of spleen (2000-fold) but the final specific activities were of a similar magnitude. 2. The liver and spleen enzymes had maximum activity at pH4.8 whereas the intestinal enzyme showed an optimum at pH5.8. The Km values for both starch and maltose with spleen and liver enzymes were higher than for the intestinal enzyme. With the intestinal enzyme, the Vmax. values were higher for both starch and maltose than those of the spleen and liver enzymes. 3. Gel filtration on Sephadex G-200 under identical conditions revealed that liver and spleen enzymes emerge from the columns much later than the intestinal enzyme. 4. Evidence is presented that the glucoamylase activity of the intestinal mucosa is exhibited by the maltase II fraction. 5. Tris, pentaerythritol and turanose inhibited glucoamylase from all the three tissues, but turanose inhibited the spleen and liver enzymes to a higher degree than the intestinal enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auricchio F., Bruni C. B. Purification of an acid alpha-glucosidase by dextran-gel filtration. Biochem J. 1967 Oct;105(1):35–38. doi: 10.1042/bj1050035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auricchio F., Bruni C. B., Sica V. Further purification and characterization of the acid alpha-glucosidase. Biochem J. 1968 Jun;108(2):161–167. doi: 10.1042/bj1080161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BAUDHUIN P., HERS H. G., LOEB H. AN ELECTRON MICROSCOPIC AND BIOCHEMICAL STUDY OF TYPE II GLYCOGENOSIS. Lab Invest. 1964 Sep;13:1139–1152. [PubMed] [Google Scholar]
  4. Brown B. I., Brown D. H. The subcellular distribution of enzymes in type II glycogenosis and the occurrence of an oligo-alpha-1,4-glucan glucohydrolase in human tissues. Biochim Biophys Acta. 1965 Oct 25;110(1):124–133. doi: 10.1016/s0926-6593(65)80101-1. [DOI] [PubMed] [Google Scholar]
  5. Bruni C. B., Auricchio F., Covelli I. Acid alpha-D-glucosidase glucohydrolase from cattle liver. J Biol Chem. 1969 Sep 10;244(17):4735–4742. [PubMed] [Google Scholar]
  6. DAHLQVIST A. Determination of maltase and isomaltase activities with a glucose-oxidase reagent. Biochem J. 1961 Sep;80:547–551. doi: 10.1042/bj0800547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAHLQVIST A. Rat-intestinal dextranase. Localization and relation to the other carbohydrases of the digestive tract. Biochem J. 1963 Jan;86:72–76. doi: 10.1042/bj0860072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DAHLQVIST A. Specificity of the human intestinal disaccharidases and implications for hereditary disaccharide intolerance. J Clin Invest. 1962 Mar;41:463–470. doi: 10.1172/JCI104499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAHLQVIST A., THOMSON D. L. SEPARATION AND CHARACTERIZATION OF TWO RAT-INTESTINAL AMYLASES. Biochem J. 1963 Nov;89:272–277. doi: 10.1042/bj0890272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dahlqvist A., Telenius U. Column chromatography of human small-intestinal maltase, isomaltase and invertase activities. Biochem J. 1969 Jan;111(2):139–146. doi: 10.1042/bj1110139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Eggermont E., Hers H. G. The sedimentation properties of the intestinal alpha-glucosidases of normal human subjects and of patients with sucrose intolerance. Eur J Biochem. 1969 Jul;9(4):488–496. doi: 10.1111/j.1432-1033.1969.tb00635.x. [DOI] [PubMed] [Google Scholar]
  12. Eggermont E. The hydrolysis of the naturally occurring alpha-glucosides by the human intestinal mucosa. Eur J Biochem. 1969 Jul;9(4):483–487. doi: 10.1111/j.1432-1033.1969.tb00634.x. [DOI] [PubMed] [Google Scholar]
  13. Fujimori K., Hizukuri S., Nikuni Z. Studies on acid alpha-1,4-glucosidase from bovine spleen. Biochem Biophys Res Commun. 1968 Sep 6;32(5):811–816. doi: 10.1016/0006-291x(68)90313-6. [DOI] [PubMed] [Google Scholar]
  14. HERS H. G. alpha-Glucosidase deficiency in generalized glycogenstorage disease (Pompe's disease). Biochem J. 1963 Jan;86:11–16. doi: 10.1042/bj0860011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kolínská J., Semenza G. Studies on intestinal sucrase and on intestinal sugar transport. V. Isolation and properties of sucrase-isomaltase from rabbit small intestine. Biochim Biophys Acta. 1967 Sep 12;146(1):181–195. doi: 10.1016/0005-2744(67)90085-x. [DOI] [PubMed] [Google Scholar]
  16. LEJEUNE N., THINES-SEMPOUX D., HERS H. G. Tissue fractionation studies. 16. Intracellular distribution and properties of alpha-glucosidases in rat liver. Biochem J. 1963 Jan;86:16–21. doi: 10.1042/bj0860016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. ROSENFELD E. L., POPOVA J. A. [The exopolyglucosidase (gamma-amylase) of the liver]. Bull Soc Chim Biol (Paris) 1962;44:129–139. [PubMed] [Google Scholar]
  19. ROZENFEL'D E. L., LUKOMSKAIA I. S., RUDAKOVA N. K., SHUBINA A. I. [Studies on alpha-1,4 and alpha-1,6-polyglucosidases of the living tissue]. Biokhimiia. 1959 Nov-Dec;24:1047–1053. [PubMed] [Google Scholar]
  20. Ruttloff H., Noack R., Friese R., Schenk G., Proll J. Uber polysaccharidspaltende Enzyme im Bürstensaum der Rattenmukosa unter besonderer Berücksichtigung der gamma-Amylase. Acta Biol Med Ger. 1967;19(6):831–839. [PubMed] [Google Scholar]
  21. Seetharam B., Swaminathan N., Radhakrishnan A. N. Purification and properties of alpha-amylase from monkey small intestine. Indian J Biochem. 1969 Jun;6(2):51–55. [PubMed] [Google Scholar]
  22. Swaminathan N., Radhakrishnan A. N. Distribution of alpha-glucosidase activities in human & monkey small intestine. Indian J Biochem. 1965 Sep;2(3):159–163. [PubMed] [Google Scholar]
  23. TORRES H. N., OLAVARRIA J. M. LIVER ALPHA-GLUCOSIDASES. J Biol Chem. 1964 Aug;239:2427–2434. [PubMed] [Google Scholar]

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