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. 1970 Jun;118(2):201–208. doi: 10.1042/bj1180201

Oxygenation of methane by methane-grown Pseudomonas methanica and Methanomonas methanooxidans

I J Higgins 1, J R Quayle 1
PMCID: PMC1179104  PMID: 5484663

Abstract

1. Experimental conditions have been found in which small amounts of methanol (approximately 2.5mm) accumulated when washed cell suspensions of methane-grown Pseudomonas methanica and Methanomonas methanooxidans were incubated with methane+oxygen mixtures in Warburg flasks. 2. The methanol formed could be separated completely from water by fractional distillation through glass helices followed by gas chromatography using 20% polyethylene glycol 400 on a Celite 545 support. 3. By using 18O-enriched oxygen gas the abundance of 18O in the methanol formed from oxidation of methane was measured with a Perkin–Elmer 270 combined gas chromatograph/mass spectrometer. The results showed that the oxygen in methanol was derived exclusively from gaseous oxygen in both micro-organisms. 4. Control experiments using [18O]water in incubation mixtures confirmed that there was negligible incorporation of the oxygen atom from water into methanol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anthony C., Zatman L. J. The microbial oxidation of methanol. 1. Isolation and properties of Pseudomonas sp. M27. Biochem J. 1964 Sep;92(3):609–614. doi: 10.1042/bj0920609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROWN L. R., STRAWINSKI R. J., MCCLESKEY C. S. THE ISOLATION AND CHARACTERIZATION OF METHANOMONAS METHANOOXIDANS BROWN AND STRAWINSKI. Can J Microbiol. 1964 Oct;10:791–799. doi: 10.1139/m64-100. [DOI] [PubMed] [Google Scholar]
  3. JAYASURIYA G. C. The isolation and characteristics of an oxalate-decomposing organism. J Gen Microbiol. 1955 Jun;12(3):419–428. doi: 10.1099/00221287-12-3-419. [DOI] [PubMed] [Google Scholar]
  4. Johnson P. A., Quayle J. R. Microbial growth on C-1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM-1. Biochem J. 1964 Nov;93(2):281–290. doi: 10.1042/bj0930281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LEADBETTER E. R., FOSTER J. W. Incorporation of molecular oxygen in bacterial cells utilizing hydrocarbons for growth. Nature. 1959 Oct 31;184(Suppl 18):1428–1429. doi: 10.1038/1841428a0. [DOI] [PubMed] [Google Scholar]
  6. LEADBETTER E. R., FOSTER J. W. Studies on some methane-utilizing bacteria. Arch Mikrobiol. 1958;30(1):91–118. doi: 10.1007/BF00509229. [DOI] [PubMed] [Google Scholar]
  7. Peterson J. A., Kusunose M., Kusunose E., Coon M. J. Enzymatic omega-oxidation. II. Function of rubredoxin as the electron carrier in omega-hydroxylation. J Biol Chem. 1967 Oct 10;242(19):4334–4340. [PubMed] [Google Scholar]

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