Table 1.
Summary of characteristics of RCC23, RCC23+3, RCC23+3p, and REV cells
| Cell | Transferred chromosome 3a | Telomerase activityb | hTERT mRNAc | Telomere lengthd | Life span |
|---|---|---|---|---|---|
| RCC23 | none | + | + | maintained | immortal |
| RCC23+3 | intact 3 | − | −e | shortens | mortal |
| progressively | (41 PDs)f | ||||
| RCC23+3p | 3pter-q22 | − | −e | shortens | mortal |
| progressively | (28 PDs)f | ||||
| REV | 3pter-p23 | + | + | maintained | immortal |
| 3cen-q22 |
a
Transferred by means of microcell fusion.
b
TRAP (telomeric repeat amplification protocol) assay.
c
Conventional reverse transcription (RT)-PCR and quantitative real-time RT-PCR (Taqman) assays.
d
Terminal restriction fragment length by Southern blot.
e
At least 64-fold lower expression than RCC23 in Taqman assay.
f
Senesce at 41 or 28 population doublings after microcell fusion.