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In S. cerevisiae W303-1A cytochrome c is released from mitochondria to the cytosol during PCD induced by acetic acid. CytC was detected by immunodetection in mitochondria (5, 10, and 20 μg of protein) and postmitochondrial supernatants obtained from S. cerevisiae stationary untreated cells (A) or cells treated with 140 mM acetic acid (B).
