Overexpression of SCD5 partially suppresses the actin organization defect of clathrin-deficient yeast. A GAL1:: CHC1 strain (SL554) was transformed with YEp24 (A, B, E, and G) or YEp24-SCD5 (C, D, F, and H). Precultures were grown on selective C-URA + 2% galactose. Then cells were washed and resuspended in medium containing galactose (A, C, E, and F) or glucose to deplete clathrin HC (B, D, G and H) for 15 h at 30°C. Cells were then fixed and stained with Alexa-568 phalloidin (A–D) or anti-actin antibodies (E–H). Bar, 5 μm. (I) Graph showing the percentage of cells from the above cultures with large patches or actin bars.