Abstract
1. Techniques have been developed to measure the concentrations of the ribonucleotides of the immature rat uterus in vivo. Tissue was frozen rapidly in liquid nitrogen, ground to a fine powder, dispersed in frozen perchloric acid and thawed slowly. Nucleotides were separated from other acid-soluble constituents on short columns of polyethyleneimine-cellulose and the mixture was resolved into individual nucleotides by two-dimensional thin-layer chromatography on polyethyl-eneimine-cellulose plates. 2. The nucleotides of immature rat uterus consisted of approximately 75% of ATP–ADP, 10–12% each of GTP–GDP and UTP–UDP and less than 2% of CTP. 3. Injection of oestradiol (5μg) promoted a linear decrease in the amounts of purine nucleotides to approximately 60% of control values in 4–5h, followed by a return to greater than control values in 8–10h. Concentrations of the pyrimidine nucleotides remained constant for 4–6h and then increased to 200% of control at 12h after hormone treatment.
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Selected References
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