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. 2002 Aug;13(8):2760–2770. doi: 10.1091/mbc.02-04-0051

Figure 1.

Figure 1

SSE1 is a multicopy suppressor of ydj1-151 temperature sensitivity and interacts with YDJ1. (A) Map of chromosome XVI in S. cerevisiae depicting the approximate positions of the DNA inserts that rescued ydj1-151 thermosensitivity, all of which included SSE1. (B) Ydj1-151 cells were transformed with a multicopy YEp24 yeast vector either 1) lacking or 2) containing YDJ1, 3) pJG8.1 (original complementing clone), or 4) pJG816 (SSE1) were struck onto SC-ura medium and were incubated at the indicated temperatures for 4 d. The growth of two transformants from each strain is shown. (C) Serial dilutions of W303 wild-type parental yeast (a), ACY17b ydj1-151 cells (b), JGY014a sse1Δ cells (c), or yeast deleted for SSE1 and containing the ydj1-151 allele (d and e) were spotted onto complete medium and incubated at the indicated temperatures for 4 d.