Figure 2.

SSE1 partially suppresses the ppαF translocation defect in ydj1-151 yeast. Ydj1-151 yeast containing either pJG816 or a vector lacking insert were labeled with [³⁵S]methionine and chased for the indicated times at either 26 or 37°C, and ppαF and BiP were immunoprecipitated from cell extracts as described in MATERIALS AND METHODS. The positions of ppαF that migrates similarly to in vitro translated ppαF in the first lane and pαF are indicated. BiP serves as a control for the efficiencies of the radiolabeling and immunoprecipitation in this experiment. The decrease in ppαF intensity over time arises from translocation and intracellular degradation of the accumulated material.