Figure 9.

The peripheral accumulation in dynamitin expressing cells requires an intact MT network. In cells overexpressing dynamitin-GFP (a) or dynamitin, few incoming capsids reach the nucleus, and many accumulate in the peripheral cytoplasm 3 h after infection. If such cells are infected in the absence of MTs, incoming capsids are distributed randomly over the entire cytoplasm, and there is no peripheral accumulation (b). Immunofluorescence microscopy of PtK₂ cells transfected with dynamitin-GFP or GFP and 46 h later infected with HSV1 for 3 h in the presence of cycloheximide and in the absence (a) or presence (b and c) of nocodazole (ND). Cells were fixed with PFA, permeabilized with TX-100, and labeled with anti-VP5 (mAb 5C10; bottom panels).