Figure 2.

(A) Alignment of Vps5p with human (Hs) and nematode (Ce) SNX1. Sequences were aligned using the CLUSTAL program available at the embnet server (http://www.ch.embnet.org/index.html). There is extensive sequence conservation particularly over the PX domain and in the C-terminal half. Vps5p is larger than human or nematode SNX1 with unconserved sequences at the N-terminal end of the protein. (B) Schematic diagram of the truncation/deletion mutants of Vps5p and Vps17p. Vps5p was dissected using the PX domain, coiled-coil domain, and sequence homology as boundaries for making truncations.