Figure 1.

CD98 expression in SCLC cells. (A) CD98 expression in H69 SCLC cells was determined by flow cytometric analysis by using an indirect immunofluorescence technique with a mAb (4F2) to the heavy chain of CD98. (B) Dose response for binding of mAb 4F2 to H69 SCLC cells. (C) Western blot analysis of whole cell lysates that had been probed with goat anti-CD98 polyclonal antibody to show CD98 expression on H69 and H345 SCLC cells, native CHO cells, and CHO cells that had been transiently transfected with CD98. (D) CD98 and β1 integrin coimmunoprecipitate in SCLC cells. H69 SCLC whole cell lysates were incubated with antibodies against CD98 (4F2) (lane 1), transferrin receptor (CD71) (lane 2), β1 integrin (K20) (lane 3), and an IgG₁ negative control antibody (lane 4), as described in MATERIALS AND METHODS. Immunoprecipitates were Western blotted and probed for β1 integrin. All data shown are representative of two to three independent experiments.