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FACS analysis of fl-HA uptake in SK-175HARE cells mediated by the 175-kDa HARE. SK-Hep-1 cells transfected with vector alone (A) or SK-175HARE-34 cells (B and C) were grown to confluence in six-well tissue culture plates, washed, and preincubated at 37°C, as indicated in the figure, with no addition or nonlabeled HA (B) or mouse IgG or mAb-174 (C) followed by fl-HA as described in MATERIALS AND METHODS. The same five conditions were used in A.
