Figure 3.
Northern blot analysis of rat LEC RNA with 175-kDa HARE cDNA probes. Total RNA (lanes 1) or mRNA (lanes 2) samples prepared from isolated rat LECs were subjected to formamide gel electrophoresis, transferred to nylon membranes, and processed as described in MATERIALS AND METHODS. The membranes were allowed to hybridize separately with three different 32P-labeled DNA probes, 5′RACE#11 (A), ZAP1P3 (B), or ZAP9P3 (C), which are located at the 5′ end, middle, or 3′ end of the 175-kDa HARE cDNA sequence, respectively.