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. 2002 Aug;13(8):2869–2880. doi: 10.1091/mbc.02-01-0004

Figure 4.

Figure 4

Activation of the HOG pathway is not required for neck localization of Ssk2p. The ssk2Δ strain TYYD5B was transformed with (A) plasmid pTY112L, expressing GFP-Ssk2pT1460A, or (B) plasmid pTY113L, expressing GFP-Ssk2pK1295N. (C) ssk1Δ strain TYY7D and (D) pbs2Δ strain TYY1B were transformed with plasmid pTY112L expressing wild-type GFP-Ssk2p. Cells were osmotically stressed with 0.9 M NaCl for 15 min, and localization of the GFP-Ssk2p fusion proteins was examined by fluorescence microscopy.