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. 2002 Aug;13(8):2869–2880. doi: 10.1091/mbc.02-01-0004

Figure 5.

Figure 5

The septin cytoskeleton is involved in neck localization of Ssk2p. (A) Wild-type (WT) strain (FY86) and cdc12–6 strain (CS01B) expressing GFP-Ssk2p from plasmid pTY111U were osmotically stressed with 0.9 M NaCl for 20 min at 25°C (left) and for 20 min after a shift to 37°C (right). GFP-Ssk2p localization was examined by fluorescence microscopy. (B) Strain Y190 was transformed with constructs encoding fusions of actin (pDab7), the septins Cdc10 (pTY123) or Cdc11 (pTY122), and Snf1 (pSE1112) to the GAL4 DBD. Strain Y187 was transformed with constructs encoding fusions of the ssk2-AIR (pMF5), ssk2-AIR ΔLD (pTY121), or Snf4p (pSE1111) to the GAL4 AD. Diploids carrying both DBD and AD fusions were selected on SC-Trp-Leu medium, then plated onto SD + 10 μg/ml adenine plus 100 mM 3-aminotriazole (Sigma Chemical Co.) to assess activation of the HIS3 reporter.