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. 2002 Aug;13(8):2869–2880. doi: 10.1091/mbc.02-01-0004

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Copyright © 2002, The American Society for Cell Biology

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Ssk2p promotes cell cycle completion after osmotic stress. Wild-type (WT) strain (FY86) and congenic deletion strains (ssk2Δ, TYYD5B; ssk22Δ, TYY3D; ssk1Δ, TYY7D; ssk2Δ/ssk22Δ, TYY9B) were synchronized by HU treatment and osmotically stressed with 0.9 M NaCl. (A) The percentages of large-budded cells in the wild-type and deletion strains before osmotic shock (time 0) vs. 60, 90, 120, and 150 min after osmotic shock are plotted. (B) The percentages of large-budded cells in ssk2Δ strain expressing GFP-Ssk2p (pTY111L), GFP-Ssk2p^T1460A (pTY112L), GFP-Ssk2p^K1295N (pTY113L), and GFP-Ssk2ΔLD (pTY119L) before osmotic shock (time 0) vs. 60, 120, 150, and 180 min after osmotic shock are plotted. n ≥ 100. Averages were calculated from 2–5 independent experiments.