Figure 5.

Effect of pH on release of HGF from stretched satellite cells. Medium was analyzed from stretched and unstretched control satellite cell cultures after 2-h treatment in DMEM beginning at 12 h postplating. Each lane represents proteins from a constant number of cultured satellite cells (1500 cells). Top, immunoblots show the effect of pH on HGF release into medium in stretched cultures. Bottom, immunoblots from unstretched control culture conditioned medium. The first lane in the top panel shows molecular weight standards, and the first lane in the bottom panel shows a control with pH 7.2 stretched medium and no first antibody.