Figure 8.

Immunoblot detection of eNOS and nNOS in 12 h satellite cell cultures. Molecular weight standards are shown in the first lane. Bands corresponding to eNOS (*) and nNOS (**) are indicated next to lanes of respective positive controls (PC1, human endothelial cell lysate; PC2 rat pituitary lysate). Three separate 12 h satellite cell culture lysates were analyzed for each form of NOS, cell lysates 1, 2, and 3 are designated under each lane. CNT represents blot of lysate 1 without primary antibody.