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NFκB activation by c-DDP. 293T cells were treated with c-DDP (20 μg/ml) at the indicated times. Nuclear extracts were prepared, and EMSA was performed as described in MATERIALS AND METHODS. Lanes 10–12 represent competition of nuclear extracts obtained after stimulation with c-DDP for 3 h with p50, p65-specific antibodies, or cold NFκB consensus oligonucleotide (κB), respectively. Arrows show the migration of different NFκB complexes.
