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. 2002 Aug;13(8):2933–2945. doi: 10.1091/mbc.E02-01-0022

Figure 9.

Figure 9

(a) Inhibition of HDAC activity does not affect the p65-dependent reporter gene expression. WT fibroblasts were cotransfected with a 5X-Gal-luc reporter plasmid (0.25 μg) and expression vectors encoding GAL4-p65 TAD1 (0.25 μg) and MEKK1 (0.5 μg). Sixteen hours after transfection cells were treated with TSA. Luciferase activity was measured 8 h later as in Figure 7. (b) Expression of p300/CBP does not increase MEKK1-induced transcriptional activation of p65. WT fibroblasts were cotransfected with a 5X-Gal-luc reporter plasmid (0.25 μg) and expression vectors encoding GAL4-p65 TAD1 (0.25 μg), MEKK1 (0.5 μg), and CBP (0.5–2 μg), as indicated. Luciferase activity was determined as in Figure 7. Data shown in this figure represent the mean of a single experiment performed in triplicate ±SD and are representative of at least three experiments with similar results.