Abstract
1. The mode of action of flax-seed hydroperoxide isomerase was studied in vitro by using as substrates linoleic acid hydroperoxides formed by soya-bean lipoxygenase. 2. The enzyme converts only 13-hydroperoxyoctadeca-cis-9-trans-11-dienoic acid, whereas the 9-hydroperoxy isomer does not react. 3. The isomerization product was identified by chemical and spectroscopic methods as 13-hydroxy-12-oxo-octadec-cis-9-enoic acid. 4. 12,13-Epoxyoleic acid isomers do not act as intermediates in the isomerization reaction. 5. Suggestions for a functional relationship between hydroperoxide isomerase and lipoxygenase are discussed.
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Selected References
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- Veldink G. A., Vliegenthart J. F., Boldingh J. Proof of the enzymatic formation of 9-hydroperoxy-10-trans, 12-cis-octadecadienoic acid from linoleic acid by soya lipoxygenase. Biochim Biophys Acta. 1970 Feb 10;202(1):198–199. doi: 10.1016/0005-2760(70)90235-3. [DOI] [PubMed] [Google Scholar]
- Veldink G. A., Vliegenthart J. F., Boldingh J. The enzymic conversions of 13-hydroperoxy-cis-9-trans-11-octadecadienoic acid into 13-hydroxy-12-oxo-cis-9-octadecenoic acid. Biochem J. 1968 Dec;110(4):58P–58P. doi: 10.1042/bj1100058pa. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Veldink G. A., Vliegenthart J. F.G., Boldingh J. Oxygen transfer in the enzymatic conversion of 18O-labelled linoleic acid hydroperoxide into the 12-keto-13-hydroxyoctadec-cis-9-enoic acid. FEBS Lett. 1970 Apr 2;7(2):188–190. doi: 10.1016/0014-5793(70)80153-3. [DOI] [PubMed] [Google Scholar]
- Zimmerman D. C. A new product of linoleic acid oxidation by a flaxseed enzyme. Biochem Biophys Res Commun. 1966 May 25;23(4):398–402. doi: 10.1016/0006-291x(66)90740-6. [DOI] [PubMed] [Google Scholar]