Figure 5.

The photo‐induced features of BAMs. a) The absorption/emission spectra of BAM14. b) The absorption/emission spectra of BAM10. c) The fluorescence of BAM14 (left) or BAM10 (right) after labeling of 15‐LOX‐1. Emission spectra for the supernatant (yellow) and resuspended pellet (cyan) after acetone precipitation. A blank sample of acetone is represented with black (left). Dot‐blot (fluorescence) of BAM10‐treated 15‐LOX‐1, with or without preincubation with ThioLox. Control experiments in the absence of 15‐LOX‐1 is shown in the bottom. Experiments were performed in duplicates (n=2). d) Emission spectra of BAM14 in presence of 5 and 20 equivalents of Fe³⁺. e) Absorption spectra of BAM12 showing the conversion from the E to Z conformation upon increasing exposure time of UV light, and return to the E conformation after heat treatment. f) Reversible photochromism of BAM12 (5 cyclic irradiations). g) Inhibitory potency of BAM12 before and after UV irradiation. h) Inhibitory potency of BAM13 before and after UV irradiation. All IC₅₀ experiments were performed in triplicates (n=3), and the standard error is reported. i) SDS‐page reveals increasing crosslinked protein species, after incubation of BAM13 (or DMSO: C) with cell lysate (with overexpressed 15‐LOX‐1) and application of UV irradiation for 0, 10, 20 and 30 min (L: protein ladder).