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TBZF and TBZ17 preferentially bind to DNA sequences containing A/G- and C/G-hybrid motifs. A, A/G- and C/G-hybrid motifs and their mutated motif sequences (mA/G and mC/G) used for electrophoretic mobility shift assay (EMSA). The mutated nucleotides are underlined. The sequences used were 60 bp, tandemly repeated four times. B, EMSA of the recombinant TBZF and TBZ17 proteins. The positions of the DNA-protein complexes are indicated by the arrowhead.
