Fig. 5.

Cell adhesion is mediated by CNTN2 (contactin‐2) in HEK293F cells. (A) A diagram of the CNTN2 constructs with GFP (green) generated for cell adhesion assays. (B) Confocal images of subcellular localization of CNTN2 full length, CNTN2 lg1‐4, CNTN2 lg1‐6, and CNTN2 FNIII domains. The confocal experiments were conducted in duplicate (Scale bar: 10 μm). (C) Cell–cell adhesion was monitored by microscopy. HEK293F cells were transfected with CNTN2 full length, CNTN2 lg1‐4, CNTN2 lg1‐6, CNTN2 FNIII domains to form cell clusters (Scale bar: 100 μm). (D) Aggregation percent; the relative percentage of the 4 cells, 10 cells were classified compared to control as a cluster (Average ± SEM, n = 10). The experiments were conducted in duplicate, with a minimum of 500 cells counted from each data point. One‐way ANOVA was employed to compare the wild‐type (WT) group and the mutant groups for the counts of 4 cells and 10 cells, respectively. The structural figures were generated utilizing UCSF Chimera software.