Clustal W (1.8) alignment (Thompson et al.,
1994) of the deduced amino acid sequences of the two annotated
peptide deformylase genes from Arabidopsis (AtDEF1, cDNA
accession no. AF250959, predicted translation accession no.
AAD39667.1, BAC F9L1, gene no. 34; and AtDEF2, cDNA
accession no. AF269165, predicted translation accession no. CAB87633.1,
BAC T15N1, gene no. 150) with the Escherichia coli peptide
deformylase (accession no. CAA54826.1). For the plant peptide
deformylases, the predicted transit peptides are highlighted in green.
The three conserved, active-site motifs including the metal binding
ligands (Meinnel, 2000) are highlighted in blue and red, respectively.
Conserved amino acids in all putative peptide deformylases (Meinnel,
2000) are in bold.