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In vitro chloroplast uptake and processing of AtDEF1. Phosphorimage analysis of an electroblotted (polyvinylidene fluoride membrane) SDS-PAGE (15%, w/v) of l-[35S]Met-labeled AtDEF1 incubated with isolated and intact pea chloroplasts for 0, 15, and 30 min (as labeled) and a 30-min import followed by 20 min thermolysin (0.1 mg mg chlorophyll−1) treatment (30 min + T). Only the processed lower molecular mass form (pAtDEF1) of AtDEF1 was protected.
