Figure 3.

Immunoreactive proteins in Arabidopsis leaf and chloroplast stromal extracts detected with antibodies raised against AtDEF1 and 2. Purified recombinant proteins (100 ng) and proteins from Arabidopsis leaves and chloroplasts (250 μg) were electrophoresed, electroblotted to a polyvinylidene fluoride membrane, and probed with primary antibody (A and C, right one-half AtDEF1 serum; and B and C, left one-half AtDEF2 serum). Pea mitochondrial and Arabidopsis chloroplast stromal proteins (250 μg) were similarly analyzed (D) with a mitochondrial-specific Vitis vinifera isoenzyme 1 NADH-Glu-dehydrogenase primary antibody (provided by K.A. Roubelakis-Angelaki). M, Prestained molecular mass markers; DEF1 and DEF2, 100 ng affinity-purified pAtDEF1 and 2; At, Arabidopsis leaf protein extract; At Cp, Arabidopsis chloroplast stromal protein extract; Ps Mito, pea mitochondrial protein extract.