Figure 4.

Affinity purification of pAtDEF1 and 2. Proteins from induced bacterial cells harboring constructs designed to produce pAtDEF1 (A) and 2 (B) were electrophoresed (15% [w/v] SDS-PAGE) and the gel stained with Coomassie blue R-250. M, Molecular mass markers; S and I, soluble and insoluble proteins, respectively, from the lysate of IPTG-induced BL21(DE3) pLysS cells expressing respective construct; E, dialyzed elution from nickel-nitrilotriacetic acid agarose column. Given below their respective gels, the N-terminal sequence for each processed form (pAtDEF) after purification from a bacterial lysate confirmed N-terminal processing.