Affinity purification of pAtDEF1 and 2.
Proteins from induced bacterial cells harboring constructs designed to
produce pAtDEF1 (A) and 2 (B) were electrophoresed
(15% [w/v] SDS-PAGE) and the gel stained with Coomassie blue
R-250. M, Molecular mass markers; S and I, soluble and insoluble
proteins, respectively, from the lysate of IPTG-induced BL21(DE3) pLysS
cells expressing respective construct; E, dialyzed elution from
nickel-nitrilotriacetic acid agarose column. Given below their
respective gels, the N-terminal sequence for each processed form
(pAtDEF) after purification from a bacterial lysate
confirmed N-terminal processing.