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. 2001 Sep;127(1):150–158. doi: 10.1104/pp.127.1.150

Table I.

Analysis of the epoxidation state in Lhca-deficient antisense plants

Time after High-Light Treatment 0 Min 5 Min 60 Min
WT 0.99 0.89 0.55
-a2 0.99 0.93 0.61
-a3 0.97 0.89 0.55

Dark-adapted wild-type (WT) and Lhca2 (-a2) and Lhca3 (-a3) antisense plants were illuminated with a PPFD of 700 μmol m−2 s−1. Leaves were taken from four plants, pooled, and frozen in liquid nitrogen 0, 5, and 60 min after high-light treatment. The pigment content was analyzed by HPLC and epoxidation state was calculated as (V + A/2)/(V + A + Z), where A, V, and Z denote concentrations of antheraxanthin, violaxanthin, and zeaxanthin, respectively.