Table I.
Time after High-Light Treatment | 0 Min | 5 Min | 60 Min |
---|---|---|---|
WT | 0.99 | 0.89 | 0.55 |
-a2 | 0.99 | 0.93 | 0.61 |
-a3 | 0.97 | 0.89 | 0.55 |
Dark-adapted wild-type (WT) and Lhca2 (-a2) and Lhca3 (-a3) antisense plants were illuminated with a PPFD of 700 μmol m−2 s−1. Leaves were taken from four plants, pooled, and frozen in liquid nitrogen 0, 5, and 60 min after high-light treatment. The pigment content was analyzed by HPLC and epoxidation state was calculated as (V + A/2)/(V + A + Z), where A, V, and Z denote concentrations of antheraxanthin, violaxanthin, and zeaxanthin, respectively.