Figure 3.

RNA blot analyses of selected H₂O₂-induced genes. a, Northern analysis of a calmodulin (accession no. R30557): lanes 1 and 2, total RNA from control and H₂O₂ (20 mm, 1.5 and 3 h pooled)-treated cells; lanes 3 and 4, mRNA from control and H₂O₂-treated cells, respectively. b, Control northern blot: RNA used in a was hybridized to EF1α cDNA to confirm equal loadings. c, RNA dot-blot analysis of calmodulin in control (C) and H₂O₂ (500 μm, H)-treated cells at 0.02 g fresh weight cells mL⁻¹. d, RNA-blot analyses of an hsp (accession no. AA042551): row A, time course analysis; lane 1, control; lanes 2 through 4, cells treated with H₂O₂ (20 mm) for 0.5 , 1.5, and 3 h. Row B, Dose response; lane 1, control; lanes 2–4, cells treated with 5, 10, and 20 mm H₂O₂ for 1.5 and 3 h (pooled RNA). Row C, Expression in rosette leaves: lane 1, leaves mock infiltrated with water; lanes 2 through 4, leaves vacuum infiltrated with 20 mm H₂O₂ for 0.5, 1.5, and 3 h.