Table 3.
Percentage of predicted thermophilic uncultivated prokaryotes without cultivated close relative, and range of predicted Topt values, in various phyla considered in this study
| Phylum | Total sequences of uncultured organisms in RDP database | Percentage of uncultured thermophiles with no close cultured relative | Examples of diverse predicted Topt values of uncultured members |
|---|---|---|---|
| Aquificae | 63 | 25.4 | Clone BH81 (AF352537), Near Boiling Silica-Depositing Thermal Springs: 86°C. VC2. 1 Bac16 (AF068794), Mid-Atlantic Ridge hydrothermal vent: 55°C |
| Cyanobacteria | 196 | 5.6 | Clone S M2A08 (AY293403), Travertine Terraces at Yellowstone Hot Springs: 60°C. SM2A09 (AY195603), Travertine depositional system at Mammoth Hot Springs: 48°C |
| Deinococcus-Thermus | 32 | 12.5 | Thermus sp. clone bacteriap1 (AF402971), Kuirau Park: 71°C. Clone PK19 (AY555779), Bor Khlueng Hot Spring in Thailand: 58°C |
| Thermomicrobia | 11 | 100.0 | Clone F BP267 (AY250872), McMurdo Dry Valleys, Antarctica: 79°C. a2b029 (AF419669), hydrothermal sediments in the guaymas Basin: 50°C |
| Thermotogae | 8 | 75.0 | Clone R5p5 (AF 482446), granular sludge: 48°C. TTA_B141 (AY297987), thermophilic anaerobic reactor degrading terephthalate: 59°C |
| Thermodesulfobacteria | 3 | 67.0 | Thermodesulfobacterium group OP B45 (AF027096), Yellowstone hot spring: 71°C |