Table I.
Levels of specific RiPAL transcripts in different organs
| Organ |
RiPAL Transcript
Levels
|
Ratio RiPAL1:RiPAL2 | |
|---|---|---|---|
| RiPAL1 | RiPAL2 | ||
| ng mg−1 total cellular RNA | |||
| Leaves | 14 | 3 | 4.4 |
| Shoots | 169 | 15 | 11.5 |
| Roots | 126 | 39 | 3.3 |
| Flowers I | 37 | 13 | 2.9 |
| Flowers II | 8 | 23 | 0.3 |
| Flowers III | 76 | 73 | 1.0 |
| Fruits I | 426 | 75 | 5.7 |
| Fruits II | 73 | 15 | 4.9 |
| Fruits III | 65 | 65 | 1.0 |
| Fruits IV | 133 | 78 | 1.7 |
| Fruits V | 84 | 20 | 4.2 |
The absolute levels of the two RiPAL transcripts were first determined for stage III fruits. The levels of transcripts of each cDNA in other organs were then determined based on the relative ratio (target:competitor) of expression of each cDNA in those tissues compared with that seen in stage III fruits (Fig. 4B).