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I-V relationships in isoosmotic (200 mm sorbitol), hypotonic (50 mm sorbitol), or hypertonic (600 mm sorbitol) solutions. I-V curves from oocytes expressing EcHKT1 (A; n = 6), EcHKT2 (B; n = 8), EcHKT1 and EcHKT2 (C; n = 7), and HKT1 (D; n = 7). Solutions also contained 1.8 mm CaCl2, 10 mm MES-Tris, 200 mm sorbitol, 1 mm Na+, and 10 mm K+ (pH 6.5).
