TABLE 2.

Identifiers to discriminate between the different ABC families and domains

ABC family	Sequencea	Blast searchb	IDc	Notes
ABCA	IGVCPQxDILW	++	+	DILW part is less conserved
	DEPSTGxDPxxRR	++	++	Overlap with the Walker B motif
	IILTTMSMEEA	+++	+++	Gives a few hits with bacterial ABC
ABCB	GKSTVLxLIxRFYDP	+++	+++	Overlap with the Walker A motif
	ANAHEFI	++	+++	Gives a few hits with bacterial ABC
	DEATSALDxESE	++	++	Overlap with the Walker B motif; gives a few hits with bacterial ABCs
ABCC-1	DLTEIGERGINLSGG	+++	+++	Overlap with the ABC signature
	DDPLSAVDxHVGxHLF	+++	+++	Overlap with the Walker B motif
ABCC-2	EKIGIVGRTGAGKSS	+++	+++	Overlap with the Walker A motif
	SVGQRQLLCL	+++	+++	Overlap with the ABC signature
ABCD	GCGKSSLFRILGGLWP	++	+++	Overlap with the Walker A motif; gives some hits with other ABCs
	TLRDQIIYPDS	++	+++
	HRxSLWKYH	++	+	Gives some hits with unrelated proteins
ABCE-1	GKSTALKILAGKxKPNLGRY	+++	+++	Overlap with the Walker A motif
	DEPSSYLDVKQRLKAAQVLRSLL	+++	+++	Overlap with the Walker B motif
ABCE-2	LNVSYKPQKIxPK	++	++	Gives some hits with unrelated proteins
	HDFIMATYLAD	+++	++	Gives some hits with unrelated proteins
ABCF-1	GRRYGLXGXNGXGKST	+++	+++	Overlap with the Walker A motif; gives some hits with bacterial ABCs
	FLNxVCTxIIH	+++	++
ABCF-2	DSRIALVGPNGAGKST	+++	+++	Overlap with the Walker A motif; gives some hits with bacterial ABCs
	DEPTNHLDIEXIDAL	+++	+++	Overlap with the Walker B motif; gives some hits with bacterial ABCs
ABCG-1	LVLGRPGxGCSTLLK	+++	+++	Detects only the ABCG-1 domain from the fungal group; overlap with the Walker A motif
	DTxVGNExxRGxSGGERKR	+++	+++	Overlap with the ABC signature; specific for domain G-1
ABCG-2	GKTTLLDVLAxRKTxG	+++	+++	Overlap with the Walker A motif; specific for domain G-2
	GLSVEQRKRLTIGVELVAKP	+++	+++	Overlap with the ABC signature; specific for domain G-2
ABCG half	IXTIHQPR	++	++	Also detects domain G2 from yeast ABCs; will not detect Dictyostelium ABCG20
ABCH	PXQLSGGEQQRV	+ (see note)	++	Overlap with the ABC signature; will detect bacterial permease.
ArsA	GGKGGVGKTTxSCS	+++	+++	Overlap with the Walker A motif
	FDTAPTGHTLRLL	+++	+++	Overlap with an unusual Walker B motif

^aHighly conserved amino-acids are boldfaced. The unusual cysteine in ABCG-1 is underlined.

^bSequences can be used with “the search for short nearly exact matches” from NCBI, and the result was checked for preferentially sorting the specified ABC domain and given a rating from + to +++.

^cEffectiveness in identifying the family for a known ABC domain; rated from + to +++.