FIG. 2.

Light regulation of TB3 expression in C. trifolii. Mycelia were grown overnight in the dark in YPSS medium and harvested by filtration. The mycelial pads were cut in half and either illuminated (L lanes) for 15, 30, or 60 min or kept in the dark (D lanes). Light and dark treatments were terminated by quick-freezing the mycelial pads in liquid nitrogen. (A) Northern blots of total RNA (30 μg/lane) prepared from C. trifolii mycelia after light exposure, with TB3 as a probe. The 25S ribosomal DNA fragment from C. gloeosporioides was used to show equivalent RNA loading. (B) Western blots of lysates (35 μg of total protein per lane) prepared from the same treated mycelia as in panel A. TB3 protein was detected with TB3 antibody.