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Immunofluorescence microscopy of TB3. Hyphae were fixed and dual stained with TB3 antibody coupled to anti-rabbit immunoglobulin G conjugated to Texas Red. Hyphae were observed by using DIC and by fluorescence microscopy (Texas Red). Nuclei were visualized with DAPI. Arrows indicate the nuclear localization of the TB3 proteins. The lack of equivalent Texas Red staining is presumably due to antibody inaccessibility to these nuclei. Bars, 10 μm.
