Figure 3.

PRMT5 KD decreases expression of genes important to stem cell growth and decreases expression of important cancer pathways. A, Differential expression analysis following PRMT5 KD in four DMG and one PHGG cell lines; the genes shown are 6 of the 11 most highly differentially regulated genes in the assay (N = 3 for all data points, and error bars not shown were too close to the central estimate to display). B, Dot plot of GSEA NES and −log(FDR) following PRMT5 KD. The DMG results represent the aggregate of four cell lines (BT245, DIPG4, DIPG7, and DIPG13), and NES reflects the magnitude of depletion. C, GSEA NES values for gene sets important for PHGG inception and growth following PRMT5 KD (FDR <0.05 for all NES values). NES, normalized enrichment score.