TABLE 2.

Oligonucleotides used in this study

Primer	Sequencea	Restriction site
BEMIF	5′-GTGGACACAGAGGTCATTC-3′
BEMIR	5′-CTGGACCTCTCGTTGTAGC-3′
HISIF	5′-TCAAGTTTGTCGGAGGCTC-3′
HISIR	5′-CCAGAATGTCACTAGCACC-3′
BEMITAG-5F	5′-GCGGTACCTCGTCAACGCAGAGCTGGAGGACGGCTC-3′	KpnI
BEMITAG-5R	5′-CCGGGCCCGACTAGCGTACAAAACTAGCTTGTTCTTTCCGTAC-3′	ApaI
BEMITAG-3F	5′-CCGGGCCCGGTAAGAGTGGCCTTTGGGAGTGCTGGAGTCAAAT-3′	ApaI
BEMITAG-3R	5′-CGGATCCAATCTCTCGGGCTCATGCATTAATCATGCA-3′	BamHI
GFP-ApaF	5′-TTAGGGCCCTGAGTAAAGGAGAAGAACTTTTCACTGGAG-3′	ApaI
GFP-ApaR	5′-TTAGGGCCCTTATTTGTATAGTTCATCCATGCCATGTGT-3′	ApaI

^aUnderlined nucleotides correspond to restriction sites.