FIG. 3.

Vegetative phenotypes in the presence of an air-water interface. (A) Growth of colonies on solid medium. Strains with the indicated genotypes were grown on VM plates at room temperature under constant light and photographed when the Δgna-1 colony had reached the edge of the plate (approximately 36 h). Wild-type colonies are approximately two times larger than Δgna-1 colonies under these conditions (data not shown). (B) Standing liquid cultures. Strains were inoculated into 2-ml portions of liquid VM and incubated in a stationary position first for 3 days at 30°C in the dark and then for 4 days at room temperature in constant light. The presence (+) or absence (−) of 2.0 mM cAMP is indicated below the test tubes.