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. 2002 Jun;1(3):481–490. doi: 10.1128/EC.1.3.481-490.2002

FIG. 1.

FIG. 1.

Repression effects of UFAs on the β-galactosidase activity of the OLE1 promoter-lacZ fusion. Constructs p62::934 and p62::396 are shown on the left. The narrow line represents the OLE1 promoter sequence. The number above each narrow line indicates the position of the deletion end point with respect to the ATG start codon of the wild-type base sequence with the A of the codon designated +1. The solid black bar represents the amino-terminal 27 amino acids of the OLE1 coding sequence fused to E. coli lacZ (blank bar). The small solid bar above the promoter indicates the FAR and LORE elements, which are also labeled. The values to the right are β-galactosidase activities under normoxic (N) and hypoxic (H) conditions, which were determined as described in Materials and Methods. The values (units of activity) shown are averages of at least three independent experiments that were performed in quadruplicate. (A) Tween 80 repression of β-galactosidase activity of reporters p62::934 and p62::396. (B) L.A. (100 μM) repression of β-galactosidase activity of reporter p62::394.