FIG. 10.

p90 Mga2p forms a complex with LORE. A biotinylated probe pull-down assay was done as described in Materials and Methods. Strain BY4741Δmga2 transformed with YEplac181-^mycMGA2 containing a myc-MGA2 fragment (myc-Mga2p) was grown under different conditions. CEs were prepared as described in Materials and Methods after incubation of yeast cells under normoxic (N) or hypoxic (H) conditions and incubated with double-stranded, biotinylated LORE (L) or mutated LORE (mL) bound to streptavidin-coated magnetic beads. Protein complexes were purified with a magnet, washed, eluted, and analyzed by Western blotting as described in Materials and Methods, except in lane 1 (no beads), for which the CE from cells grown under hypoxic conditions was used directly in the Western blot. The blot was probed with a monoclonal antibody to c-myc. The positions of the p120 and p90 forms of Mga2p are indicated.